For brand new experiments, often users will meet with staff to discuss the purpose and goal of the experiment and offer any advice for preparation or what settings on the machine may work best. Do not pour anything down the drain unless specifically directed to do so by a staff member.Į-mail Mitchell Koptchak to schedule a new sorting appointment or for questions about sorting.Assume all chemicals are hazardous, regardless of label, handle with caution. ![]() All users are required to complete laser safety training from EH&S and provide a copy of your certificate of completion to the Flow Cytometry staff as well as e-mailing a copy to All fixatives, solvents and media must be handled in a hood with caution. It is recommended that all persons working in a laboratory attend the chemical safety training seminar, a free course given by Environmental Health and Safety at Penn State. There is a zero tolerance policy for unsafe or careless handling of chemicals or equipment in the facility. The only exception is the Astrios cell sorter which can run up to BSL-2+ materials since it is housed in a bio-safety cabinet. If they are potentially infectious, or human cell lines they should be fixed before running them on any of our machines. All samples run on our machines should be BSL-1 materials. However we still have chemicals like concentrated ethanol, bleach, and potentially toxic dyes like Propidium Iodide and DAPI, so gloves should be worn when working around any potential chemical or biological hazard in the lab. There are not many chemical hazards in the Flow Cytometry Core. You will need to bring a copy or e-mail staff a copy of the training certificate you receive at the end as well as e-mail a copy to safety trainingĪll persons working in a laboratory should attend the chemical safety training seminar, a free course given by Environmental Health and Safety at Penn State. Required safety trainingĪll users must complete Penn State EH&S Laser Safety training prior to being trained on any flow cytometer. We currently do not train users on our cell sorters. Billing for training will include the equipment time, plus and additional staff training rate ($34.64/hr). Training is usually divided into several 1-2 hour sessions each encompassing specimen preparation and basic equipment use. We provide training on an individual basis. Various University safety courses are also recommended or required. We offer training on equipment use and sample preparation.
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